Medium-term Storage of Apricot Shoot Tips In Vitro by Minimal Growth Method
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چکیده
منابع مشابه
In vitro Plant Regeneration of Helianthus Annuus (Hyb. Azargol) From Alginate-Encapsulated Shoot Tips for Short Term Storage, Germplasm Exchange and Distribution
The present study demonstrates the potential of nutrient-alginate encapsulation of shoot tips of sunflower, Helianthus annuus (hyb. Azargol) for synthetic seed technology, which could be useful in germplasm distribution and exchange. Shoot tips from in vitro shoot cultures derived from mature seed explants were encapsulated in 3% sodium alginate and 100 mM CaCl2. 2H...
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Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...
متن کاملEncapsulation of Shoot Tips and Nodal Segments for in Vitro Storage of “Kober 5BB” Grapevine Rootstock
In vitro preservation of the “Kober 5BB” rootstock (Vitis berlandieri ˆ Vitis riparia) was assessed with the encapsulation technique and slow growth storage. Shoot tips and nodal segments excised from in vitro cultures were encapsulated in calcium-alginate beads. A 30 min ion exchange time proved optimal for forming proper beads. The encapsulated and naked explants were stored at 4 ̋C in the da...
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Multiple shoots were achieved through axillary bud explant of Spilanthes acmella on MS medium supplemented with 0.5mg/l BA. In vitro developed axillary buds and tiny shoots isolated from the multiple shoots were encapsulated in different strength of sodium alginate. The present work explains the role of components of culture medium on morphogenic response of encapsulated shoot tips to various t...
متن کاملCryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification
Shoot tips from 4to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week....
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ژورنال
عنوان ژورنال: HortScience
سال: 1999
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.34.7.1277